Enzymes Specificity

Enzymes Specificity
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Purpose: This experiment seeks to check the ability of lactase to specifically interact and bind to lactose as opposed to maltose.
Background
The experiment seeks to test enzyme specificity for the lactase enzyme on two separate substrates of maltose and lactose. In definition, an enzyme is a chemical catalyst that helps in speeding up a chemical reaction by reducing the required activation energy to facilitate the reaction ADDIN CSL_CITATION { “citationItems” : [ { “id” : “ITEM-1”, “itemData” : { “DOI” : “10.1002/9780470015902.a0000716.pub2”, “ISBN” : “9780470015902”, “ISSN” : “9780470015902”, “abstract” : “Speciufb01city is a hallmark of enzyme catalysis; it is inseparable from catalytic eufb03ciency, the other hallmark of enzyme reactions. Speciufb01city arises from the three- dimensional structure of the enzyme active site; this site is complementary to the transition state of the reaction. The substrate ufb01ts snugly within the enzyme active site, optimally aligned to react with the catalytic residues. In addition, the conformation of the substrate is constrained, which will control the course of the reaction.”, “author” : [ { “dropping-particle” : “”, “family” : “Hedstrom”, “given” : “Lizbeth”, “non-dropping-particle” : “”, “parse-names” : false, “suffix” : “” } ], “container-title” : “Encyclopedia of Life Sciences”, “id” : “ITEM-1”, “issued” : { “date-parts” : [ [ “2010” ] ] }, “title” : “Enzyme Specificity and …